inha (mca951s) monoclonal  (Bio-Rad)

Journal: Anticancer research

Article Title: Potential roles of activin in head and neck squamous cell carcinoma progression in epithelial-mesenchymal transition, metastasis, and mortality

doi: 10.21873/anticanres.16733

Figure Lengend Snippet: Immunohistochemistry

Article Snippet: Antibody Clone Source Dilution ACVR1B (MAB222) Monoclonal R & D Systems 1:200 ACVR2 (AF340) Polyclonal R & D Systems 1:100 ACVR2B (AF339) Polyclonal R & D Systems 1:80 INHA (MCA951S) Monoclonal Biorad 1:800 INHBA (Serotec/Biorad) Monoclonal Biorad 1:100 INHBB (Serotec/Biorad) Monoclonal Biorad 1:100 Open in a separate window Immunohistochemistry MTT assay Cell proliferation was determined by MTT incorporation.

Techniques:

Journal: Anticancer research

Article Title: Potential roles of activin in head and neck squamous cell carcinoma progression in epithelial-mesenchymal transition, metastasis, and mortality

doi: 10.21873/anticanres.16733

Figure Lengend Snippet: Immunohistochemistry

Article Snippet: INHA (MCA951S) , Monoclonal , Biorad , 1:800.

Techniques:

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling

doi: 10.1186/s12958-017-0312-z

Figure Lengend Snippet: Immunohistochemical analysis of ovarian tumor markers. a-j Expression of granulosa cell and germ cell markers in 8-week-old control and TGFBR1-CA G9Cre ovaries. Representative images from immunohistochemical analysis of FOXL2 ( a and b ), INHA ( c and d ), FOXO1 ( e and f ), AMH ( g and h ), and DDX4 ( i and j ) are shown. k and l Negative controls using isotype-matched rabbit and goat IgGs. Experiment was performed using ABC method, and signals were developed using NovaRED Peroxidase Substrate Kit. Sections were counterstained with hematoxylin. Five independent samples per group were utilized in this analysis. Scale bar is representatively depicted in ( a ) and equals 50 μm ( a - l )

Article Snippet: Following H 2 O 2 treatment and blocking, the sections were incubated with primary antibodies directed to FOXL2 (Abcam; ab5096; 1:1500), INHA (AbD Serotec; MCA951ST; 1:300), forkhead box O1 (FOXO1) (Cell Signaling; 2880; 1:400), anti-Mullerian hormone (AMH) (Santa Cruz; sc-6886; 1:2000), and DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4) (Cell Signaling; 8761; 1:500).

Techniques: Immunohistochemical staining, Expressing, Control

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Disruption of postnatal folliculogenesis and development of ovarian tumor in a mouse model with aberrant transforming growth factor beta signaling

doi: 10.1186/s12958-017-0312-z

Figure Lengend Snippet: Evidence of TGFBR1 activation in ovarian granulosa cells of TGFBR1-CA G9Cre mice. a-d Real-time PCR analysis of expression of TGFBR1 CA , Smad7 , Inha , and Zp3 in ovaries from control and TGFBR1-CA G9Cre mice at PD3 and PD7. Real-time PCR was performed using ΔΔCT method. Data are mean ± s.e.m. n = 4-5. * P < 0.05, ** P < 0.01, and *** P < 0.001. Ns, not significant. e Western blotting analysis of TGFBR1 CA , phospho-SMAD2/3, and HSD3B using ovaries from 2-month-old control and TGFBR1-CA G9Cre mice. TGFBR1 CA was detected using an anti-HA antibody. n = 3. Each lane represents an independent sample. f-i X-gal staining using ovaries from Rosa26 / Gdf9 -iCre mice ( f - h ) and Rosa26 control mice ( i ). Panels g and h are higher magnification images of two different fields of panel ( f ). At least 3 independent samples per group were used. j Real-time PCR analysis of the expression of Gli1 , Gli2 , and Tgfbr3 using ovaries from 8-week-old control and TGFBR1-CA G9Cre mice. Data are mean ± s.e.m. n = 4-5. * P < 0.05 and *** P < 0.001. k-n RNAscope in situ hybridization analysis of Gli1 mRNA distribution using 8-week-old control ( k ) and TGFBR1-CA G9Cre ovaries ( l ). n = 4. Positive and negative controls using TGFBR1 CA flox/+ ovaries were shown in ( m ) and ( n ), respectively. Sections were counterstained with hematoxylin. Scale bar is representatively shown in ( f ) and equals 25 μm ( g , h , and k - n ) and 100 μm ( f and i )

Article Snippet: Following H 2 O 2 treatment and blocking, the sections were incubated with primary antibodies directed to FOXL2 (Abcam; ab5096; 1:1500), INHA (AbD Serotec; MCA951ST; 1:300), forkhead box O1 (FOXO1) (Cell Signaling; 2880; 1:400), anti-Mullerian hormone (AMH) (Santa Cruz; sc-6886; 1:2000), and DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4) (Cell Signaling; 8761; 1:500).

Techniques: Activation Assay, Real-time Polymerase Chain Reaction, Expressing, Control, Western Blot, Staining, RNAscope, In Situ Hybridization